Abstract
Objective To test the pathogeny of Vibrio cholerae strains of the epidemic in Jinzhou of Hubei Province. Methods Traditional methods of biochemistry, immobilization test, string test and typing of blood serum were used to test the 6 strains isolated. The virulence gene which was cholera enterotoxin (ct) was detected by PCR. The whole genome DNA finger print of confirmed strains was analyzed by pulsed field gel electrophoresis (PFGE) after digestion respectively with two enzymes Not Ⅰ and Sfi Ⅰ. The DNA fingerprints were analyzed for clusters. Results The 6 strains of Vibrio cholerae were all O139 by traditional laboratory tests; virulence gene was detected in all 6 strains. The banding pattern was the same in the two maps of PFGE. The results of cluster analysis showed that the similarity coefficient of the six strains was 100%. Conclusion The epidemic of Vibrio cholerae is caused by the same pathogenic bacterium which is O139. Key words: Vibrio cholerae; Virulence gene; Pulse field gel electrophoresis
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