Pathogenicity of halophilicVibrio harveyiin giant freshwater prawns (Macrobrachium rosenbergiiDe Man)

Abstract

In this study, the phenotypic and pathogenic properties in freshwater host were characterized in 14 strains of halophilic Vibrio harveyi isolated from infected marine black tiger shrimp, Penaeus monodon. The lysogenic phenotype was assayed via prophage excision and mitomycin C induction. The bacteria were grouped into two types, corresponding to lysogenic and non-lysogenic strains. The pathogenicity was determined via direct injection of bacterial cultures into post-larval juvenile giant freshwater prawns, Macrobrachium rosenbergii De Man. All of the infected prawns showed similar symptoms and inflamed hepatopancreas. The V. harveyi isolates derived from the first-injected infected prawns were re-isolated and re-injected into healthy giant freshwater prawns, in which they retained similar infectivity. Both lysogenic and non-lysogenic Vibrio spp. showed identical virulence associated with 100% mortality within one day post-injection. TEM micrographs showed hepatopancreatic nuclear deformation and lipid breakdown caused by lysogenic γ-hemolytic VL19 and non-lysogenic β-hemolytic V33. However, the V33 strain was associated with severely disrupted mitochondria. None of the V. harveyi strains was able to produce a biofilm. Together, our findings indicate that the lysogenic and non-lysogenic halophilic V. harveyi isolated from marine shrimps may use different virulence factors that are responsible for their pathogenicity in freshwater prawns.