Pathogenesis of oncogenic simian adenoviruses: VI. An Ultrastructural Investigation of SV30 Replication

Abstract

Newly synthesized infectious simian adenovirus 30 (SV30) was detected at 12–16 hours PI by titration of infectivity in two continuous kidney cell lines. These infectivity titers reached a maximum at 30 hours. Immunofluorescent observations correlated with the ultrastructural sequence of events. Intranuclear concentric membranes formed “tubes” which contained individual “coated” virus particles arranged in a linear order. Intranuclear rectilinear bars associated with “uncoated” nucleoids were also noted. In LLC-MK 2 and Vero cell cultures infected with SV30, increased numbers of intracytoplasmic annulate lamellae were apparent.