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Abstract
SummarySummaryNovelty: Very large probes with a high complexity are used in the fluorescence based detection of chromosomal abberations, such as genetic rearrangements involving chromosomes 3, 13 and/or 17. These rearrangements are related to conditions retinoblastoma, osteoblastoma, lung cancer and breast cancer.Biology: The probes are up to 50 kb in length and can be of several different DNA sequences to provide internal controls for the correct chromosome. The probes can be propagated in YACs or lambda vectors. The probes are hybridized to interphase and metaphase chromosomes with reliable signals and detected via fluorescence.Chemistry: The probes are subjected to PCR amplification and labelled with biotin. A texas red avidin probe was used to detect the PCR products using a fluorescence microscope. Specific structural and numerical aberrations can be detected.
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