Abstract
This paper describes the determination of the unidirectional fluxes of [ 3H]3- o-methylglucose, [ 14C] d-glucose, [ 14C]mannitol and [ 14C]urea across the isolated retina pigment epithelium of the frog, Rana catesbiana, placed as a membrane between two well-stirred fluid compartments. The data indicated that in preparations with a d.c.-resistance of 100–200 ohm cm 2 there was a statistically significant difference between the influx (choroidal to apical) of 3- o-methylglucose and of d-glucose and the flux of these compounds in the opposite direction. No differences were detected for the fluxes of mannitol and urea. The level of electrical resistance of the preparation did not affect the apical to choroidal flux of [ 3H]3- o-methylglucose whereas higher resistances decreased the flux in the direction towards the retina. The data is discussed in terms of the sugar requirements of the retina.
Published Version
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