Abstract
Publisher Summary The partition affinity ligand assay (PALA) method is normally used as a nonequilibrium method, that is, the binding reaction involved does not have to reach equilibrium. A requirement for a binding assay in aqueous two-phase systems is that the reactants in the binding reaction must have markedly different partition coefficients. The PALA principle includes incubation of the reaction mixture and subsequent separation with an added phase system, both steps being carried out in a single test tube. No separate washing steps and centrifugations are needed. In a direct binding assay, the binding between two different molecules is utilized to quantify one of them. Sufficiently concentrated aqueous solutions of some water-soluble polymers, forming phase systems, can be used for selective distribution of macromolecules. Such phase systems have a top phase rich in one of the polymers and a bottom phase rich in the other. Compared to the phase systems generally used in chemistry, that is, those containing organic solvents, such water-rich systems have been shown to be better for separating biological material.
Published Version
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