Abstract

This protocol describes the use of the Spectradyne nCS1 instrument to measure the particles per mL concentration and size of nanoparticles. The Spectradyne nCS1 is a particle-analyzing instrument that uses microfluidic resistive pulse sensing, rather than optical measurements, to determine the size and concentration of samples. The size and concentration of a sample are determined by measuring the changes in voltage as particles travel through a nano-constriction in the microfluidic cartridge. This method also has the advantage over optical techniques in that measurements are not dependent on the type of material being measured (e.g., refractive index of the sample itself is not needed for accurate analysis), and only microliters (typically 5μL) of a sample are needed for analysis.

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