Participation of kinin system on CMC pouch inflammation (author's transl)

Abstract

The role of kinins and their related substances in inflammatory process was studied by employing carboxymethylcellulose (CMC) pouch method in a rat. Kinin and kininase activities, pH value, and leucocyte migration in the effusion from experimental CMC pouch were measured, and kininase and kininogen levels in plasma were also determined. An increase of kinins in the effusion was found from 3 to 4 hr after CMC administration, and the content in the 3 hr effusion was higher than that at 4 hr, while an increase of leucocyte migration became significant at 4 hr. From these results, it seems plausible that the kinins found in effusion were not derived from migrated leucocytes but from plasma, because plasma kininogen decrease was found in inflammatory rat. Potent kininases were still found in the effusion in this investigation, and the activity in the effusion elevated parallel with kinin activity in it. Though it has been reported that pH value of inflammatory effusion was acidic and kininases were inactivated in acidic condition, the effusion from CMC-induced inflammation was found to be neutral. It has been widely accepted that kinins might be one of the chemical mediators in inflammatory processes, but the present results seem to indicate that the kinin system may participate only in the very early stage of the processes.