Partial Substitution of K by Na Alleviates Drought Stress and Increases Water Use Efficiency in Eucalyptus Species Seedlings.

Nikolas De Souza Mateus,Antônio Leite Florentino,Elcio Ferreira Santos,Alexandre De Vicente Ferraz,José Lavres,José Leonardo De Moraes Goncalves

doi:10.3389/fpls.2021.632342

Nikolas De Souza Mateus, Antônio Leite Florentino + Show 4 more

Open Access

https://doi.org/10.3389/fpls.2021.632342

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Abstract

Eucalyptus, the most widely planted tree genus worldwide, is frequently cultivated in soils with low water and nutrient availability. Sodium (Na) can substitute some physiological functions of potassium (K), directly influencing plants’ water status. However, the extent to which K can be replaced by Na in drought conditions remains poorly understood. A greenhouse experiment was conducted with three Eucalyptus genotypes under two water conditions (well-watered and water-stressed) and five combination rates of K and Na, representing substitutions of 0/100, 25/75, 50/50, 75/25, and 100/0 (percentage of Na/percentage of K), to investigate growth and photosynthesis-related parameters. This study focused on the positive effects of Na supply since, depending on the levels applied, the Na supply may induce plants to salinity stress (>100 mM of NaCl). Plants supplied with low to intermediate K replacement by Na reduced the critical level of K without showing symptoms of K deficiency and provided higher total dry matter (TDM) than those Eucalyptus seedlings supplied only with K in both water conditions. Those plants supplied with low to intermediate K replacement by Na had improved CO2 assimilation (A), stomatal density (Std), K use efficiency (UEK), and water use efficiency (WUE), in addition to reduced leaf water potential (Ψw) and maintenance of leaf turgidity, with the stomata partially closed, indicated by the higher values of leaf carbon isotope composition (δ13C‰). Meanwhile, combination rates higher than 50% of K replacement by Na led to K-deficient plants, characterized by the lower values of TDM, δ13C‰, WUE, and leaf K concentration and higher leaf Na concentration. There was positive evidence of partial replacement of K by Na in Eucalyptus seedlings; meanwhile, the ideal percentage of substitution increased according to the drought tolerance of the species (Eucalyptus saligna < Eucalyptus urophylla < Eucalyptus camaldulensis).

Highlights

The genus Eucalyptus plays an important role in meeting the growing global wood demand (Paquette and Messier, 2010)
Abaxial stomatal density (StdAB) was influenced by Na and water stress (WS) in E. saligna and E. urophylla, whereas in E. camaldulensis, it was affected by Na application (Figures 1D– F)
E. saligna and E. camaldulensis seedlings grown under WW and WS conditions showed higher adaxial stomatal density of leaf surface (StdAD) and abaxial stomatal density of leaf surface (StdAB) levels with partial K replacement by Na

Summary

Introduction

The genus Eucalyptus plays an important role in meeting the growing global wood demand (Paquette and Messier, 2010). It is largely dependent on fertilization (Smethurst, 2010) and vulnerable to drought, the main limiting factors for plant growth (Gonçalves et al, 2017). Stomatal closure by osmotic adjustment (Oddo et al, 2011) is a key factor to mitigating the negative impacts of drought, avoiding excessive water loss at the expense of photosynthetic rate restrictions (Anjum et al, 2011) and turgor loss, decreasing cell growth (Steudle, 2000). The ion flux in and out of the guard cells mediates stomatal aperture and closure (Kim et al, 2010)

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