Abstract
Proteases are the main enzymes responsible for the breakdown of protein molecules into amino acids. These enzymes can easily be obtained from plants, animals and microorganisms. However, the easiest, fastest and cheapest source is microorganisms. Bacillus cereus strains B16, B17, B18, B19 and B20 were selected for use in this study. The optimum conditions and other parameters of the strains in the production process of proteases were investigated. The strains were characterized using biochemical methods. Different culture conditions were analyzed for the production efficiency of protease activity. Based on the proteolytic activity analysis, Bacillus cereus B17 strain was selected for detailed studies. Protease purification was done by dialysis, ion exchange chromatography and gel filtration chromatography. It was determined that the growth and the protease activity of Bacillus cereus B17 was greatly affected by parameters such as pH and temperature of the medium, presence of metal ions and inhibitors, and incubation time.
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