Abstract
Mucoran, an acidic heteropolysaccharide isolated from the cell walls of the fungus Mucor rouxii, was purified by DEAE-Sephadex chromatography. It consists mainly of D-glucuronic acid, D-mannose, and L-fucose in a 5:3:2 ratio plus small proportions of galactose and glucose. Mucoran was subjected to methylation by the Hakomori procedure. Only about 60–70% of the polysaccharide was recovered as fully methylated material. A large proportion of this methylated material was dialyzable, indicating extensive depolymerization, probably via β-elimination, during exposure to dimethylsulfinyl carbanion. The fully methylated fraction of mucoran (both dialyzable and nondialyzable portions) consists of unbranched glucuronomannan chains, with equal proportions of 4-linked D-glucuronic acid residues and 3-linked D-mannose residues. The aldobiouronic acid, α- D-glucopyranosyluronic acid-(1→3)- D-mannose, was a major product of partial acid hydrolysis of mucoran. The principal structural feature of mucoran is the following alternating sequence of D-glucuronic acid (GlcA) and D-mannose (Man) residues: D-Man-(1→[4)-α- D-GlcA-(1→3)- D-Man-(1] n-.
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