Partial argentation resin chromatography (PARC): II. Separation of saturated and Mono‐, Di‐, Tri‐ and tetraenoic fatty esters

Abstract

AbstractPartial argentation resin chromatography (PARC) was used to separate a mixture of saturated and monoenoic, dienoic, trienoic and tetraenoic fatty esters. A series of columns containing XN1010 sulfonic acid resin silvered in the range of 17~91% of theoretical (meaning 17~91% of the sulfonic acid protons were replaced by silver ions) were prepared and were used to correlate the percentage silver with the separation and the retention times of unsaturated fatty esters.cis‐Trienoic and tetraenoic fatty esters were eluted with methanol on PARC columns containing 36 and 17% silver. Partial silvering of the resin improved peak shapes while sample elution time and elution volume were reduced. Application of PARC to the separation of mixtures of fatty acids and mixtures of triglycerides was investigated and found to be not feasible with the present system.