Partial argentation resin chromatography (PARC): III. the effects of sodium ion incorporation and solvent on the separation of mixtures of fatty acids, of fatty esters, and of triglycerides

Abstract

AbstractPartial argentation resin chromatography (PARC) was used to separate mixtures of fatty acids, of fatty esters and of triglycerides. Various eluting solvents (methanol, acetone, ether, benzene) were investigated and acetone was the preferred solvent. To prevent acid‐catalyzed condensation of the acetone, the sulfonic acid protons of the XN1010 resin were replaced with sodium ions before silver ion incorporation. Atomic absorbtion spectrophotometry was used to determine the number of silver ions incorporated per volume of resin. A mixture containing stearic, elaidic and oleic acids was separated on a saturated silver resin column. A commercially available sample of linoleic acid was purified on a 53% PARC column (meaning 53% of the available sulfonic acid protons in the resin were replaced by silver ions). A 39% PARC column was used to separate a mixture of linseed acids. Methyl linoleate, linolenate and arachi‐donate also were isolated on a 39% PARC column. A mixture containing triolein, trilinolein and trilinolenin was separated on a 32% PARC column.