Parenteral nutrition leads to alteration of hepatocyte cell cycle? Gone expression in rats

Abstract

Objective Total parenteral nutrition (TPN)-associated liver disease (PNALD) is a well-recognized complication. Despite the severity of this disease, neither the etiology nor the patho-physiology of PNALD is currently understood. The goal of these experiments was to determine changes of cell cycle genes, using real time PCR array. Methods 12 male SD rats were randomized into two groups. The TPN group (N=6) received standard TPN solution through a silastic catheter inserted in the right jugular vein and similarly the control group (N = 6) received the physiologic saline. After 7 days of infusion,we analyze the difference of cell cycle gene expressions between the two groups with real time PCR array. Results The TPN group was found to have large lipid droplets with unclear mar-gins in the perilobular region and smaller lipid droplets in the centrilobular regions. Nine genes were up-regulated in the TPN group: Atm, Brca1, Cdkn1b, Dnajc2, G2a , Nfatc1, Notch2, Pkd1, Ppp2r3a. Seven genes with down-regulated in the TPN group: Cdc25 b, Cend 1, E2f 1, Mcm3, Mcm2, Nek2, Wee1. Conclusions This study demonstrates significant ahemations in gene expressions in liver of rats which received TPN. These alterations may provide insight into potential mechanism of TPN-induced hepato-cyte injury. Key words: Parenteral nutrition, Total; Hepatocytes; Gene expression chips