Abstract
Polyploidy is a common phenomenon that has played a significant role in the evolutionary history of angiosperms. The allopolyploid Gossypium species (AD-genome) is the result of hybridization between two diploid species (A-genome and D-genome). In order to further explore the possible A- and D-genome donor of extant tetraploid cotton species, two molecular markers (random amplified polymorphic DNA [RPAD] and simple sequence repeat [SSR]) were employed on 13 cotton species including nine diploid (two A-genome and seven D-genome) and four tetraploid cotton species (AD-genome). Genomic in situ hybridization (GISH) was also used to further confirm the genomic origin and organization of tetraploid cotton species. The result showed that 26 of 40 RAPD primers and 49 of 120 SSR primers were polymorphic by 65% and 40.8% respectively. Clustering analyses for RAPD and SSR results indicated that the genome of G. raimondii is the nearest to the D-subgenome of tetraploid species, and it may be the ancestral D-genome do...
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