Abstract
Background. Obesity and dietary habits are associated with increased incidences of aging-related prostatic diseases. The present study was aimed to investigate transgenerational effects of chronic high-fat diet (HFD) feeding on inflammation and senescence-related changes in prostate. Methods. Sprague-Dawley rats were kept on either normal or HFD one. Senescence-associated β-galactosidase (SA β-gal) activity, inflammation, and cellular proliferation were determined in the prostate. Results. Increased SA β-gal activity, expression of p53, and cell proliferation marker PCNA were observed in ventral prostate of HFD-fed rats. Immunostaining for p53 and PCNA revealed that the p53 immunopositive cells were primarily in stroma while PCNA immunopositive cells were epithelial cells. An increase in expression of cycloxygenase-2 (COX-2) and phosphorylation of nuclear factor-kappa B (NF-kB) was observed in prostate of weaning pups HFD-fed parents. However, in adult pups, irrespective of dietary habit, a significant increase in the expression of COX-2, PCNA, phosphorylation of NF-kB, infiltration of inflammatory cells, and SA β-gal activity was observed. Conclusions. Present investigation reports that HFD feeding promotes accumulation of p53 expressing cells, proliferation of epithelial cells, and senescence-related changes in prostate. Further, parental HFD-feeding upholds inflammatory, proliferative, and senescence-related changes in prostate of pups.
Highlights
Obesity has crossed the epidemic proportion in several countries, leading to an explosion of obesity-related health disorders
The pups of the high-fat diet (HFD)-fed parents were subdivided into two groups and one group continued on HFD and other on normal pellet diet (NPD) (HNPD)
ventral prostate (VP) of HFD-fed rats showed enhanced activity of SA β-gal as compared to that of age-matched NPD-fed control (Figure 2(a)), which is indicative of higher lysosomal mass during replicative aging
Summary
Obesity has crossed the epidemic proportion in several countries, leading to an explosion of obesity-related health disorders. Sedentary lifestyle, and diet rich in fat are probably the most important factors for the high incidence of metabolic diseases, diabetes, and prostatic disorders. Obesity and dietary habits are associated with increased incidences of aging-related prostatic diseases. Increased SA β-gal activity, expression of p53, and cell proliferation marker PCNA were observed in ventral prostate of HFD-fed rats. An increase in expression of cycloxygenase-2 (COX-2) and phosphorylation of nuclear factor-kappa B (NF-kB) was observed in prostate of weaning pups HFD-fed parents. In adult pups, irrespective of dietary habit, a significant increase in the expression of COX-2, PCNA, phosphorylation of NF-kB, infiltration of inflammatory cells, and SA β-gal activity was observed. Present investigation reports that HFD feeding promotes accumulation of p53 expressing cells, proliferation of epithelial cells, and senescence-related changes in prostate. Parental HFD-feeding upholds inflammatory, proliferative, and senescence-related changes in prostate of pups
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