Abstract
ABSTRACTGlioblastoma (GBM) is one of the most lethal brain cancers worldwide, and there is an urgent need for development of novel therapeutic approaches. Parecoxib is a well-known cyclooxygenase-2 (COX-2) inhibitor, and had already been developed for postoperative analgesia with high efficacy and low adverse reaction. A recent study has suggested that parecoxib potently enhances immunotherapeutic efficacy of GBM, but its effects on GBM growth, migration and invasion have not previously been studied. In the present study, MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] and BrdU (5-bromo-2-deoxyuridine) incorporation assays were used to evaluate the cell proliferation of GBM cells. Wound-healing and transwell assays were preformed to analyze GBM cell migration and invasion, respectively. The results suggested that parecoxib inhibits cell proliferation, migration and invasion of GBM cells in a dose-dependent manner. RT-qPCR (real-time quantitative PCR) analysis demonstrated that miRNA-29c can be significantly induced by parecoxib. Furthermore, our data suggests that a miRNA-29c inhibitor can significantly attenuate parecoxib's effect on proliferation, migration and invasion of GBM. In conclusion, the present study suggests that parecoxib inhibits GBM cell proliferation, migration and invasion by upregulating miRNA-29c.
Highlights
Glioblastoma (GBM) is a grade IV glioma classified by the World Health Organization (WHO), which is one of the most lethal and aggressive brain cancers, and accounts for 15% of brain malignancies (Young et al, 2015)
These results indicated that parecoxib inhibits the cell proliferation of GBM cells
In the present study, we found that parecoxib can inhibit cell proliferation, migration and invasion of GBM cells, and identified that miRNA-29c is an important miRNA that is potentially implicated in the inhibitory effect of parecoxib on GBM cell proliferation, migration and invasion
Summary
Glioblastoma (GBM) is a grade IV glioma classified by the World Health Organization (WHO), which is one of the most lethal and aggressive brain cancers, and accounts for 15% of brain malignancies (Young et al, 2015). The typically treatment for GBM involves surgery, chemotherapy, radiotherapy or combination therapy. The therapies for GBM have largely improved in the past few decades, the survival rate of patients with GBM is still low, as less than approximately 5% of patient survive more than five years (Gallego, 2015). There is an urgent need to explore and develop new therapeutic approaches for prevention and treatment for the deadly malignancy.
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