Parasite and bacterial co-infections with Leishmania spp. in dogs

Abstract

Canine visceral leishmaniasis (CVL) is a major disease affecting dogs and is often associated with other illnesses. In this study, we investigated the distribution of helminths, ectoparasites and bacteria in dogs of an endemic urban area of CVL. A total of 71 dogs, uninfected or naturally infected with Leishmania spp. were studied. Splenic samples were cultured for Leishmania identification, and anti-Leishmania antibodies were measured in the serum. Helminths were diagnosed in the feces using flotation or spontaneous sedimentation techniques. Serum antibodies against six ectoparasite-transmitted pathogens were detected. Microbial growth from eyes, skin, urine, and blood samples were evaluated. To our knowledge, this is the first time that co-infections with Leishmania spp., parasites and bacteria together has been reported. Co-infections with Leishmania were observed in 89% of the animals with helminths and 95% with ectoparasites. Most of the dogs were positive for Ehrlichia spp. and Anaplasma spp. Coagulase-negative Staphylococcus was the most frequently isolated organism. It is found that Leishmania positivity dogs from endemic area in Brazil have a higher rate of co-infections with helminths, ectoparasites and bacteria. Therefore, effective treatment and public measures are needed to contain the spread of canine leishmaniasis and other infections.