Parapiptadenia rigida MYCORRHIZATION WITH SPORES OF Scleroderma citrinum

Gerusa Pauli Kist Steffen,Rosana Matos De Morais,Joseila Maldaner,Cleber Witt Saldanha,Ricardo Bemfica Steffen,Táscilla Magalhães Loiola

doi:10.1590/01047760201723022342

Gerusa Pauli Kist Steffen, Rosana Matos De Morais + Show 4 more

Open Access

https://doi.org/10.1590/01047760201723022342

Copy DOI

Journal: CERNE	Publication Date: Jun 1, 2017
Citations: 1	License type: cc-by

Affiliation: Universidade do Estado de Santa Catarina

Abstract

ABSTRACT Ectomycorrhizal fungal inoculation in forestry seedlings aids plant establishment and growth in the field. The objectives of this study were: to determine the mycorrhizal capacity of the ectomycorrhizal fungus Scleroderma citrinum in Parapiptadenia rigida (red angico) seedlings and to evaluate the viability of a mycorrhizal inoculation technique for forest seedlings involving the use of spores. Mature spores were inoculated in the substrate (75% soil and 25% carbonized rice husk), totaling 1.5 grams of fungal spores per liter of substrate. P. rigida seeds were sown in substrates inoculated or not inoculated with fungal spores in presence or absence of Pinus echinata and Eucalyptus citriodora essential oil: not inoculated (T1), inoculated (T2), inoculated more pine essential oil (T3), inoculated more eucalyptus essential oil (T4). Seedlings of Pinus elliottii were used for a positive control of mycorrhizal inoculation (T5) and not inoculated (T6) with fungal spores. At 90 days after sowing, the base stem diameter, height, fresh and dry weight of shoots and roots, percentage of root colonization and Dickson Index were determined. The presence of fungal structures in P. rigida and P. elliottii roots inoculated with S. citrinum spores was observed, demonstrating the occurrence of an ectomycorrhizal association. The application of pine and eucalyptus essential oils in the substrate increased the percentage of ectomycorrhizal colonization in P. rigida seedlings. The addition of S. citrinum mature spores in the substrate used for seedling production is a viable practice for ectomycorrhizal inoculation and it can be used in forest nurseries in controlled mycorrhization programs.

Highlights

The symbiotic interaction of ectomycorrhizae with forest species is considered very beneficial, especially in areas of degraded and fragile soil, commonly found in the Pampa biome of Rio Grande do Sul, Brazil (NARA; HOGETSU, 2004).The need for mycorrhization of forest species was demonstrated for the first time by Frank (1894), the first records of mycorrhizae are from 1840, when R
The objectives of this study were to determine the mycorrhizal capacity of fECM S. citrinum in P. rigida seedlings and to evaluate the viability of the ectomycorrhizal inoculation technique in forest seedlings using spores removed from mature sporocarps of S. citrinum
Seeds of P. rigida and P. elliottii were obtained from the forest seed bank in the Forest Research Center of Fundação Estadual de Pesquisa Agropecuária (Santa Maria, Rio Grande do Sul state, Brazil), where they were stored in a cold room at 8 °C and 55% relative humidity

Summary

Introduction

The symbiotic interaction of ectomycorrhizae with forest species is considered very beneficial, especially in areas of degraded and fragile soil, commonly found in the Pampa biome of Rio Grande do Sul, Brazil (NARA; HOGETSU, 2004).The need for mycorrhization of forest species was demonstrated for the first time by Frank (1894), the first records of mycorrhizae are from 1840, when R. Among the several genera of ectomycorrhizal fungi (fECM), Scleroderma, Hysterangium and Rhizopogon stand out as important biotechnological resources to be used in forestry (SULZBACHER, 2010), due to the benefits that the ectomycorrhizal association provides in plants. These benefits include increased water and nutrient absorption capacity, a higher tolerance to abiotic stresses (salinity, drought and presence of heavy metals) and suppression of pests and plant diseases (CARDOSO FILHO et al, 2016). The ectomycorrhizal character of Scleroderma sp. has been known for a long time (GURGEL et al, 2008; SOYTONG et al, 2014), little is known about the diversity and the association ability of species of this genus with host plants in tropical regions and, mainly, in native forest environments in South America and Brazil (GURGEL, 2009)

Objectives

Methods

Findings

Conclusion