Selection of the optimal composition of vegetable oil and chlorophyllipt oil components

O A Katsaraba,R M Sachuk,S V Zhyhalyuk,Yu V Horyuk,Ya S Stravsky

doi:10.32718/ujvas2-3.03

Abstract

Investigate the antimicrobial properties of various concentrations of vegetable essential oils in combination with an oil solution of chlorophyllipt as possible ingredients for ointments for wounds. Microbiological tests were performed according to standard methods using E. coli test cultures, S. aureus, Str. agalactiae and P. fluorescens. The results of studies of antibacterial activity of both individual ingredients and their combinations are presented, namely: 4 % essential oil of Siberian pine, 2 % essential oil of eucalyptus, 1.5 % essential oil of cloves, 1.5 % of essential oil of cedar, 2.0 % tea tree essential oil and 1.0 % chlorophyllipt oil solution. Bacteria, which are usually typical agents of wound infections, have been found to be quite sensitive to the drugs tested. High activity of essential oils and oily solution of chlorophyllipt with respect to E. coli and S. aureus was revealed. In particular, a 1.0 % oily solution of chlorophyllipt caused staphylococcal growth retardation zones whose diameters were 1.4 times larger than the antibiotic clindamycin. It was determined that representatives of gram-negative microflora were more sensitive to the investigated essential oils and chlorophyll. The optimal composition of the experimental drug called “Ointment for wounds” is offered. The results of preclinical testing showed a sufficiently high efficiency compared to traditional means. The results obtained with regard to antimicrobial activity indicate the prospect of using preparations based on the essential oil of Siberian pine, eucalyptus, carnation, cedar, tea tree and oil solution of chlorophyllipt for the treatment of skin diseases in animals. This data will help to develop new effective and safe veterinary treatments for wound care.

Highlights

The pharmaceutical market of veterinary dermatological medicines of Ukraine is filled mainly with preparations of foreign production
Increasing the concentration of the preparations from 1.5 to 2.0 % for the essential oil of tea tree, from 4 % for pine anthrax and from 2.0 % for eucalyptus, carnation, cedar contributes to the increase in the level of antistaphylococcal activity of the studied samples, as well as to broadening the spectrum their antimicrobial action (Table 1)
Used as a sample comparison with the antibiotic ointment clindamycin was effective against Escherichia coli, the growth retardation zone was 24.0 ± 0.71 mm; moderately effective for staphylococcus (19.3 ± 1.08 mm) and streptococcus (18.7 ± 0.41 mm), ineffective for the causative agent of pseudomonosis (13.7 ± 2.48 mm)

Summary

Introduction

The pharmaceutical market of veterinary dermatological medicines of Ukraine is filled mainly with preparations of foreign production. The trend of recent times is the use of biologically active components of plant origin as an alternative to antibiotic therapy (Tamminen et al, 2018; Vorobets et al, 2018). Selected combinations of essential oils are often no less effective than synthetic antibiotics, and the risk of resistant strains of microorganisms is reduced (Queiroga et al, 2018; Santos et al, 2019). Herbal remedies can include a combination of many compounds that best meet the intended therapeutic goals (Okmen, et al, 2016). The combination of antimicrobial, anti-inflammatory, immunomodulatory and reparative properties is the main criterion for the selection of biologically active substances of plant origin (Wolski et al, 2017). The above pharmacological characteristics include Siberian pine essential oil (Shpatov et al, 2017; Carrión-Prieto et al, 2018), eucalyp-

Methods

Results

Conclusion