Parameters of antioxidant activity of Galega officinalis L. and Galega orientalis Lam. (Fabaceae Lindl.) plant raw material

Dzhamal Rakhmetov,Jan Brindza,Oksana Shymanska,Eva Ivanišová,Olena Vergun,Olga Grygorieva

doi:10.5219/1271

Abstract

The plant raw material of Galega officinalis L. (goat’s rue) and Galega orientalis Lam. (fodder galega) investigated in this study. These species are known as fodder crops with high productivity of green mass and as medicine plants. The current study was aimed to evaluate an accumulation in dry raw of selected plants the total content of phenolic acids (TPA) and flavonoids (TFC) as compounds with antioxidant activity (AA) by spectrophotometric method. AA by DPPH-method and phosphomolybdenum method (reducing power (RP)) was measured. Study of ethanolic extracts of G. officinalis showed accumulation of TPA in different organs in range from 3.65 to 15.17 mg.g-1 caffeic acid equivalent (CAE) and TFC from 10.08 to 65.75 mg.g-1 quercetin equivalent (QE), AA by DPPH-method from 6.02 to 8.45 mg.g-1 Trolox equivalent (TE) and RP of extracts by phosphomolybdenum method from 86.56 to 288.15 mg TE.g-1. In extracts of G. orientalis was identified TPA from 3.52 to 18.52 mg CAE.g-1 and TFC from 6.09 to 46.72 mg QE.g-1, antioxidant activity by DPPH-method from 6.80 to 8.48 mg TE.g-1 and antioxidant capacity by phosphomolybdenum method from 52.52 to 188.51 mg TE.g-1. It was established that less concentration of studied compounds found in the stems for both species. It should be noted that the content of phenolic acids in the leaves was decreased and flavonoids in stems increased during vegetation for both species. Content of phenolic acids in the generative organs and flavonoids in the leaves decreased in raw of G. orientalis during vegetation. Pearson’s correlation analysis demonstrated very strong relations between TFC and AA by DPPH, TPA and RP, TFC and RP for G. officinalis extracts. Very strong correlation in the extracts of G. orientalis found between TFC and RP, TPA and RP. Obtained results can be used in the further biochemical and pharmacological study.

Highlights

Study of the antioxidant activity and compounds that cause it very widespread and in modern biological science (Carocho and Ferreira, 2013; Kumar, Sharma and Vasudeva, 2017)
Based on the results obtained in this study concluded that two investigated species of Galega L. as medicine and forage cultures characterized by plant raw material with high antioxidant activity
The maximal content of phenolic acids for both investigated species was found in the leaves in the period at the inflorescence emergence, flavonoids in inflorescences at the flowering stage for G. officinalis and in the leaves at the inflorescence emergence period for G. orientalis

Summary

Introduction

Study of the antioxidant activity and compounds that cause it very widespread and in modern biological science (Carocho and Ferreira, 2013; Kumar, Sharma and Vasudeva, 2017). Leguminous plants (Fabaceae Lindl.) are a perspective group of crops, which ecological and economic function is important in agriculture. It is one of the most important plant families in the production of food for humans and livestock, as well as in the production of industrial products. Plants from the Fabaceae family are of interest in relation to biologically active compounds, especially individuals, in different organs (Danilčenko et al, 2017)

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