Abstract
Abstract Specific characteristics of Paragonimus westermani, P. ohirai , and P. miyazakii were sought in electrophoretic patterns of whole body proteins. From P. westermani , a minimum of 23 distinct protein bands was separated. Densitometric tracings gave at least 6 prominent peaks considered to be species specific. A minimum of 24 distinct bands was identified from P. ohirai . At least 7 of the bands were prominent and considered characteristic of P. ohirai . No significant difference was found in the electrophoretic patterns and the R F value of each band separated from P. ohirai worms obtained from rats and cats. A minimum of 23 distinct bands was identified from P. miyazakii . This species has at least 6 prominent bands, of which two (bands 7 and 11) were especially high in density. There was observed no significant difference between P. miyazakii worms originating from crabs collected at Iwakuni or at Kurodasho. The three species of lung flukes showed similar electrophoretic patterns in the gel regions near the origin and the front band. Two prominent bands identified from these gel regions were common to all of the above species. The major differences are found in the mid region of the gel. Each of the three lung flukes has a species-characteristic electrophoretic pattern containing several prominent protein fractions. These patterns are highly reproducible and definitive for all of the species.
Published Version
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