Abstract
An enzyme-free electrochemical immunoassay is introduced into microfluidic paper-based analytical devices for the first time using novel three dimensional flower-like Au nanoparticles modified paper working electrode (FLAuNPs-PWE) as sensor platform and gold (Au)-silver (Ag) bimetallic nanoparticles (Au-Ag BMNPs) as tracer. FLAuNPs with large surface area serve as an effective matrix for primary antibodies. Au-Ag BMNPs with good biological activity, such as mimicking natural peroxidases, are used for binding of signal antibodies (Ab2). Then the Ab2 labeled BMNPs is linked to the FLAuNPs-PWE via sandwich immunoreactions. Enhanced sensitivity is achieved by efficient catalysis of the Au-Ag BMNPs toward hydrogen peroxide. Under the optimized experimental conditions, the proposed paper-based electrochemical immunosensor exhibits excellent analytical performance for enzyme-free detection of carcinoembryonic antigen, ranging from 0.001-50ngmL−1 with a low detection limit of 0.3pgmL−1. The immunosensor shows good precision, acceptable stability and reproducibility. The proposed method provides a new promising platform for the design of the highly sensitive detection method, showing great promise for clinical application.
Published Version
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