Abstract
Klebsiella pneumoniae is responsible for numerous infections caused in hospitals, leading to mortality and morbidity. It has been evolving as a multi-drug resistant pathogen, acquiring multiple resistances such as such as horizontal gene transfer, transposon-mediated insertions or change in outer membrane permeability. Therefore, constant efforts are being carried out to control the infections using various antibiotic therapies. Considering the severity of the acquired resistance, we developed a panel of strains of K. pneumoniae expressing different resistance profiles such as high-level penicillinase and AmpC production, extended spectrum beta-lactamases and carbapenemases. Bacterial strains expressing different resistance phenotypes were collected and examined for resistance genes, mutations and porin alterations contributing to the detected phenotypes. Using the Massive parallel sequencing (MPS) technology we have constructed and genotypically characterized the panel strains to elucidate the multidrug resistance. These panel strains can be used in the clinical laboratory as standard reference strains. In addition, these strains could be significant in the field of pharmaceuticals for the antibiotic drug testing to verify its efficiency on pathogens expressing various resistances.
Highlights
Over the last three decades, we have observed increased occurrence of multidrug-resistant Enterobacteriaceae
Bacteria identification was performed using VITEK 32 GN system (BioMérieux, Marcy l’Etoile, France), and was confirmed using the direct colony method with MALDI-TOF MS (Bruker Daltonics, Bremen, Germany). These isolates were screened for specific phenotypes such as high level acquired penicillinase, over produced AmpC, extended spectrum beta-lactam (ESBL), and carbapenemase based on Bonnet R, et al in ‘‘Antibiogram’’ (LeClercq, Courvalin & Rice, 2010) and ‘‘From antibiogram to prescription’’ book (Francois et al, 2004) from the hospital database
Among 3,000 K. pneumoniae collection, we could select eighteen isolates showing typical phenotypes i.e. six ESBL producing, nine carbapenemase producing, one isolate expressing High level penicillinase, one high level AmpC β-lactamase producing and one wild-type strain susceptible to antibiotics except ampicillin
Summary
Over the last three decades, we have observed increased occurrence of multidrug-resistant Enterobacteriaceae. These are constantly evolving as resistant pathogens posing the serious problems in the choice of an appropriate antibiotic treatment in the hospital settings (Davies & Davies, 2010). It has evolved to be multi-drug resistant, showing high resistance to extended spectrum beta-lactam (ESBL), fluoroquinolones, aminoglycosides and even the last resort ‘carbapenems’ (Fair & Tor, 2014). How to cite this article Dsouza et al (2017), Panel strain of Klebsiella pneumoniae for beta-lactam antibiotic evaluation: their phenotypic and genotypic characterization.
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