Abstract
Summary Aims A number of chemical compounds such as sodium tungstate and vanadyl sulfate have been assessed for their anti-diabetic effects in several animal models of diabetes. This study investigates the proper time for initiation of sodium tungstate treatment in diabetic Wistar rats and its histopathological effects on pancreas. Materials and methods Animals were divided into five groups ( n = 10). Control (C), sodium tungstate-treated control (TC), streptozotocin-induced diabetic (D), streptozotocin-induced diabetic rats were treated by sodium tungstate form 1 week before streptozotocin (STZ) injection (TD 1 ), and diabetic rats treated with sodium tungstate 1 week after STZ administration (TD 2 ). The histopathologic changes in pancreas were investigated by light microscopy. Body weight, blood glucose, insulin levels, food intake and fluid intake were compared between groups. Serum insulin levels were determined by ultra-sensitive rat insulin kit, using double-antibody ELISA. Results Group D and TD 2 showed significant weight loss, polydipsia, polyuria and polyphagia compared to groups C and TC ( p 1 group protects diabetic-induced rats from elevation of glucose compared to groups D and TD 2 ( p 1 showed significant decrease in fasting glucose levels and oral glucose tolerance test in comparison to groups D and TD 2 ( p 1 group, tungstate protects STZ-induced beta-cells degenerations. Conclusion Pre-treatment with sodium tungstate leads to amelioration of diabetic complications and short-term pre-treatment with tungstate can converts the diabetic state by sustaining a few, although it is critical, amount of beta-cells that adequately maintain normoglycemia.
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call