Abstract
The islet purification step in clinical islet isolation is important for minimizing the risks associated with intraportal infusion. Continuous density gradient with a COBE 2991 cell processor is commonly used for clinical islet purification. However, the high shear force involved in the purification method using the COBE 2991 cell processor causes mechanical damage to the islets. We and other groups have shown human/porcine islet purification using large cylindrical plastic bottles. Shear stress can be minimized or eliminated using large cylindrical plastic bottles because the bottles do not have a narrow segment and no centrifugation is required during tissue loading and the collection processes of islet purification. This review describes current advances in islet purification from large mammals and humans using a COBE 2991 cell processor versus large cylindrical plastic bottles.
Highlights
We and other groups have reported islet purification from large mammals and humans using large cylindrical plastic bottles [18,19,20,21,22,23,24], which substantially improved the efficacy in comparison to standard purification using a COBE 2991 cell processor [18]
This review describes current advances in islet purification from the pancreas of large mammals and humans using a COBE 2991 cell processor versus large plastic bottles
Their data suggest that the technique using the COBE 2991 cell processor allows isolation of intact, viable human islets of sufficient purity forare human islet transplantation
Summary
The standard method of islet purification is centrifugation by density gradients, as the density between exocrine tissue and islets is different. We and other groups have reported islet purification from large mammals and humans using large cylindrical plastic bottles [18,19,20,21,22,23,24], which substantially improved the efficacy in comparison to standard purification using a COBE 2991 cell processor [18]. This review describes current advances in islet purification from the pancreas of large mammals and humans using a COBE 2991 cell processor versus large plastic bottles. Med. 2021, 10, 10 the pancreas of large mammals and humans using a COBE 2991 cell processor versus large plastic bottles
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