Pancreatic cancer diagnosis by infrared serum spectroscopy

Abstract

Relevance . The problem of the diff erential diagnosis of pancreatic cancer (PCa) and acute pancreatitis remains relevant throughout the world. The available clinical, laboratory, instrumental preoperative, and often intraoperative, including morphological, data do not always allow us to accurately determine the nature of the focal lesion of the pancreas. Purpose of the study . To develop a method for the early diagnosis of pancreatic cancer by the method of infrared spectroscopy of blood serum. Materials and methods . By the method of infrared spectroscopy of blood serum, 30 patients with pancreatic disease were examined: 20 patients with acute pancreatitis, 10 patients with prostate cancer. The control group consisted of healthy volunteers (20 people) without diseases of the pancreas. The study was carried out by infrared (IR) spectroscopy of a patient’s blood sample in the region of absorption spectra of 1200–1000 cm –1 [31]. As diagnostic parameters, we chose the ratio of the heights of the peaks of the absorption bands on each other: 1165/1150 (Parameter 1), 1165/1090 (Parameter 2), 1165/1080 (Parameter 3), 1165/1070 (Parameter 4), 1165/1050 (Parameter 5). Results . The values of the parameters of the IR spectra of the blood serum of patients with PCa are signifi cantly lower than the values of the corresponding parameters in acute pancreatitis by 42% (P1), 54% (P2), 53% (P3), 59% (P4), 57% (P5), respectively (p≤0.05). Such a sharp diff erence in IR spectroscopic parameters allowed us to visualize the method and create diff erential diagnostic profi les of pancreatic diseases. Conclusions . A change in the content of phosphorus-containing compounds (ATP, ADP, AMP) in the blood during acute pancreatitis and PCa causes changes in spectroscopic characteristics. The method of IR spectroscopy allows us to diff erentiate these diseases of the pancreas. IR spectroscopic parameters allow the formation of diff erential diagnostic images of pancreatic cancer and acute pancreatitis and visualize mathematical calculations. The proposed method is non-invasive, that is, it allows without internal intervention in the patient's body to diagnose acute pancreatitis and pancreatic cancer.