Paired box 2 induces epithelial-mesenchymal transition in normal renal tubular epithelial cells of rats

Abstract

Epithelial‑mesenchymal transition (EMT) is considered to be a crucial stage of renal fibrosis. Previous studies have indicated that paired box2 (PAX2) affects renal fibrosis, likely by regulating EMT. Therefore, we examined whether PAX2 directly induces normal renal tubular EMT invitro. Recombinant PAX2 plasmid was transfected into a renal tubular epithelial cell line (NRK52E) derived from normal rats. The optimal time‑point was evaluated through fluorescence detection, western blotting and real-time polymerase chain reaction (PCR). Morphological alterations were examined using phase‑contrast microscopy. At the optimal time‑point of transfection, the expression of E-cadherin, α-smooth muscle actin (α-SMA), fibronectin and snail was analyzed by western blotting and real-time PCR. PAX2 expression in NRK52E cells transfected with PAX2 plasmid reached its peak at 72h post‑transfection. The transfected cells became elongated. PAX2 transfection induced a fibrotic phenotype of NRK52E cells with increased expression of α-SMA, fibronectin and snail as well as decreased expression of E-cadherin. The optimal time-point for transfection efficiency was 72h. In conclusion, PAX2 induces renal tubular mesenchymal transition invitro.