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Abstract
H2S has been found to act as a neuroprotectant. Cystathionine-beta-synthase (CBS) is one of three enzymes for production of H2S in mammals. The present study was conducted to investigate whether CBS expression can be affected by in utero cigarette smoke exposure (CS) in the neonatal rats. SD pregnant rats were randomly divided into control and CS groups. The neonatal offspring rats were used in experimental observation. The methylene blue assay method was used to examine H2S production rate by medulla oblongata. CBS mRNA and protein expression in medulla oblongata were analysed by RT-PCR and Western blot, respectively, and the levels of CBS expression in neurons and glia of medullary nuclei were examined with immunohistochemical technique. In medulla oblongata of CS group, compared to control group, H2S production rate was increased (P
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