Abstract
We previously revealed that cystathionine-beta-synthase (CBS) for production of H 2 S is expressed in neonatal rat medulla oblongata and CBS-H 2 S pathway contributes to protection of medullary respiratory centers against injury induced by acute hypoxia in both neonatal and adult rats. The present study was carried out to explore if expression of CBS/H 2 S in medulla oblongata is upregulated by chronic intermittent hypoxia (CIH) in adult rats. Adult SD rats were randomly divided into control and CIH groups. The levels of mRNA and protein of CBS in medulla oblongata were analyzed by RT-PCR and Western blot, respectively. The expression of CBS protein in medullary respiration-related nuclei was examined with immunohistochemistry. H 2 S production rate by medulla oblongata was measured by methylene blue assay. It was indicated that the levels of both mRNA and protein of CBS in medulla oblongata were higher in CIH group ( P P 2 S by medulla oblongata was elevated in CIH group ( P 2 S in medulla oblongata can be upregulated by CIH, suggesting that CBS-H 2 S pathway may be involved in protection of medullary respiratory centers against CIH injury in adult rats.
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