P54 Effect of hydrogen sulfide on contraction of smooth muscle of colon with slow transmit constipation

Abstract

Background Slow transit constipation (STC) is a clinical syndrome, including many gastrointestinal manifestations, such as, abdominal pain, bloating, nausea, malaise, anorectal symptoms suggestive of difficult fecal expulsion. In the scientific literature generally refers to patients who primarily complain of constipation and have delayed colonic transit but no underlying systemic disorder or pelvic floor dysfunction. The implication is that STC represents a disorder of colonic motor function. Hydrogen Sulfide (H2S) has been considered the third gaseous transmitter like nitric oxide (NO) and carbon monoxide (CO). Increasing evidence indicates that H2S acting an important role in the colon. It has dual effect on spontaneous contraction in the guinea-pig, significantly decrease in rectal compliance only with a higher dose, but failed to induce colonic smooth muscle relaxation at a low dose. H2S inhibits contractile activity of ileal longitudinal muscle, relaxants mouse distal colonic smooth muscle, and strongly inhibits in vitro intestinal and colonic motor patterns. In this study, we explore the possible relationship between H2S and the pathology of STC to determine the effects of H2S on colonic smooth muscle of STC. Methods Compound Diphenoxylate was used to set up the mice models of slow transmit constipation (STC). The colonic tissues of patients with STCs were also collected. The mice and human colonic tissues were connected with BL-420E biology function system. NaHS and L-Cysteine were taken as donors of H2S, recorded the area under the curve (AUC) of spontaneous contractions and contraction frequency after drug additional to study the contraction of colonic tissues in vitro. On the other hand, fifty-four mice were divided into 6 groups with 9 rats in each group: blank group; slow transmit constipation group; motilium group; high does treat with NaHS group; middle does treat with NaHS group; and low does treat with NaHS group. Giving different medicine to each group and count ink drive rate to estimate the effect of H2S to the intestinal movement. Results ∘1 Both NaHS and L-Cysteine have effects on spontaneous contractions of colonic muscle circle in human and mice. They can increase the spontaneous contraction of colonic smooth muscle circle in vitro in STC, and this effect is not concentration-dependent. ∘2 NaHS can increase the drive rate in STC mice, indicate that NaHS have obvious effects on intestinal movements. Conclusion The H2S has an obvious effect on colon smooth muscle contraction in STC, and can increase the intestinal movements in STC. H2S releasing drugs may have therapeutic potential in patients with STC.