P5 Gonorrhoea audit: changing pattern of antibiotic sensitivity and persistence of dna detection 2007–2014: Abstract P5 Table 1

Abstract

Background Nucleic acid amplification testing (NAAT) is widely used in GUM clinics to diagnose GC infection; its in-built high sensitivity may potentially detect DNA from non-viable organisms following successful treatment. The BASHH national guidelines stipulate that test of cure (TOC) with NAAT should take place 2 weeks post treatment. The purpose of this study was to determine whether this is an adequate time interval to perform TOC. We also analysed the changing pattern of antibiotic sensitivity between 2007–2014. Methods All GC cases at our clinic between 01/01 to 30/06 in 2007–2014 were identified and assessed for antibiotic sensitivity and TOC. Results In 2014 there were 126 cases, culture and sensitivity results were available for 85. TOC with NAAT was done in 71 cases. There were 5 cases where the NAAT was SDA positive but not PCR, two of these had a negative NAAT when tested at 29 and 57 days post treatment. Two patients DNA for a repeat NAAT. The fifth had serial repeated NAAT SDA positive results (however original cultures were sensitive to 1 st line therapy), this patient had a negative NAAT after re-treatment. There was one positive result 14 days after treatment (re-treated); the NAAT was not repeated. Overall a TOC with NAAT was performed between 7–50 days after treatment with a mean, median and mode of 18, 15 and 14 days respectively. Conclusion None of the cultures were resistant to ceftriaxone. Follow up with NAAT testing at 2 weeks appeared to be adequate for TOC.