P109 PASSIVE ECCRINE SWEAT SENSING FOR DUPLEX TEMPORAL DETECTION OF CYTOKINES

Abstract

Abstract Introduction Sweat based wearable sensors have shown a lot of promise in the recent years due to the ability of tracking the biomarkers in real-time. Among the various biomarkers present in sweat, cytokine concentrations have been shown in comparable range to the blood cytokines. Detection of sweat cytokines can help in monitoring of inflammation in real-time. In this work, we demonstrate a duplex cytokine sweat based sensor for real-time monitoring. The developed sensor can detect interleukin-6 (IL-6) and interleukin-10 (IL-10) in real-time that can aid in prognosis or recovery of inflammation. Materials and Methods The sweat based sensor was developed with semiconducting electrode on porous polymeric substrate on a porous polymer substrate. Affinity capture probes were immobilized on the sensor surface via a thiol-cross linking chemistry. ATR-IR was used to validate immobilization of the capture probes. Electrochemical impedance measurements technique was used to detect the interaction between the specific antibody and target analyte. The impedance response based on the binding interaction was used to quantify the sensor metrics. Results In this work, a sweat sensor platform for the duplex detection of IL-6 and IL-10 has been demonstrated. The conjugation of the antibodies to the sensor surface was confirmed using ATR-IR spectroscopy. Impedance response was used to characterize the sensor performance metrics. Calibration dose response curve was developed with decreasing impedance response for increasing concentrations of target analyte. A limit of detection of 2 pg/mL was obtained with a dynamic range from 2 pg/mL- 200 pg/mL for IL-6. IL-10 demonstrated a limit of detection of 1.5pg/mL with a dynamic range from 1.5- 150pg/mL. The developed sensor demonstrated minimal or no cross-reactivity to non-specific molecules. Conclusion A novel sweat-based biosensor for duplex detection of cytokine markers has been demonstrated. The developed biosensor can detect pro and anti-inflammatory cytokines that can aid in the prognosis or recovery of inflammation.