Abstract
Reactive oxygen species (ROS) are known to be involved in the maintenance of redox homeostasis and various cellular signaling pathways. Non-toxic level of ROS has been reported to be involved in the adipocyte differentiation from 3T3-L1 cells. Previous study showed that ROS is intermediately generated in pre-adipocytes and be involved in modulating pre-adipocyte differentiation. The potent inhibitory effect of carnosic acid (CA) on adipocyte differentiation was observed using Oil Red O staining assay. The ROS level reached to a maximum level at two days in adipocyte differentiation. CA attenuated the expression of NADPH oxidase 4 (Nox4) and p47phox. In addition, CA suppressed the expression of transcription factor such as C/EBPα, C/EBPβ, PPARγ, and NF-κB involved in adipocyte differentiation. Furthermore, CA enhanced the expression of phase II antioxidant enzymes through the up-regulation of Nrf2 activation. These results indicate that the inhibitory effect of CA on the differentiation of 3T3-L1 cells into adipocyte can be accomplished by regulating ROS production via Nox 4 and ROS scavenging via Nrf2 activation. Consequently, the regulation of ROS may be the crucial factor in inhibitory effect of CA on adipogenesis, further implying ROS as a prime target of obesity control.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
