P10 Protective effect of exogenous hydrogen sulfide and donepezil in PC12 cells treated with Aβ

Abstract

Objective To observe the effect of exogenous hydrogen sulfide (H 2 S) and Donepezil on surviving rate in pheochromocytoma cells (PC12 cells) treated with beta amyloid peptide 25–35 (A β 25–35). Methods PC12 cells were cultured in vitro and sulfur hydrogenated sodium (sodium hydrosulfide, NaHS) was used as exogenous H 2 S donor. The cells were divided into six groups: control group, A β 25–35 group, NaHS group, NaHS plus A β 25–35 group, Donepezil group, and Donepezil plus A β 25–35 group. The NaHS group was further divided into seven groups in different concentrations of NaHS: 10, 25, 50, 100, 200, 500, 1000 μM, respectively. Donepezil concentration was 20 μM. After treatment for 24 h, the PC12 cell survival rates were measured by MTT (3-(4,5)-dimethylthiahiazo (z-y1)-3,5-diphenytetrazoliumromide) method. Results (1) H 2 S significantly increased the survival rate of PC12 cells in vitro:the surviving rates in different NaHS (10–200 μmol/L) groups were 97.27%, 103.24%, 108.18%, 116.50% and 112.22% ( p p p β 25–35 significantly reduced survival rate of PC12 cell (46.55% vs 95.74% in control group, p p > 0.05), 25 μmol/L ∼ 200μmol/L of NaHS increased the survival rate of PC12 cell (58.96%, 62.03%, 68.56%, 64.23% vs 46.55%, respectively, p β 25–35 were concentration-dependently inhibited by NaHS from 10 μM ∼ 200 μM ( p p > 0.05, compared with 46.55%). (4) Donepezil reduced the toxic effect of A β 25–35 and increased the survival rate in PC12 cells (72.15% vs 46.55%, p Conclusion NaHS and Donepezil increase the survival rate of PC12 cells, suggesting that H 2 S exerts protective effect on PC12 cells by A β 25–35.100 μM is an optimal concentration of NaHS in cells cultured in vitro.