P023 - Investigation of Oncostatin M protein expression in Iranian relapsing remitting multiple sclerosis patients

Abstract

Background Oncostatin M (OSM) is an interlukin-6 (IL-6) cytokine secreted by diverse immune cells in-order to moderate cell proliferation, survival, differentiation, and inflammation by effecting gp-130 cell surface receptors. Relapsing remitting multiple sclerosis (RRMS) is the common type of MS influencing 85% of MS patients. It seems that up-regulation of cytokines has an undeniable role in arising autoimmune mechanisms and symptoms. Isfahan province, Iran, is an area with an alarming increase in incidence and prevalence of MS in recent years. Thus, we focused on the investigation of OSM as an immune-dependent risk factor in disease patho-physiology in Isfahan RRMS patients. Objectives To measure the association between OSM plasma level in MS patients and the healthy subjects. To evaluate the correlation between the demographic info such as age, sex, EDSS, drugs, and OSM concentration in cases. Methods Blood sample from 60 RRMS patients and 30 healthy subjects between 15 and 40 years was gathered on EDTA and the evaluation on OSM in their plasma was achieved by EASTBIOPHARMA enzyme-linked immunosorbent assay (ELISA) kit. The statistical analysis was performed utilizing SPSS software. Results Data showed a significant higher OSM plasma level in the cases than the healthy donors (P value<0.05). Conclusions As the first step of studying IL-6 cytokine family in autoimmune diseases in Iranian population, we accomplished to approve the OSM active role in RRMS patients of Isfahan province. Further future investigation of IL-6 family such as “studying IL-6 in central nervous system (CNS) plaques”, “genetic polymorphisms in association with MS”, and “revealing newer aspects of their implications in cellular pathways” might result in development of immunotherapy and diagnosis in chronic autoimmune diseases of the CNS. Oncostatin M (OSM) is an interlukin-6 (IL-6) cytokine secreted by diverse immune cells in-order to moderate cell proliferation, survival, differentiation, and inflammation by effecting gp-130 cell surface receptors. Relapsing remitting multiple sclerosis (RRMS) is the common type of MS influencing 85% of MS patients. It seems that up-regulation of cytokines has an undeniable role in arising autoimmune mechanisms and symptoms. Isfahan province, Iran, is an area with an alarming increase in incidence and prevalence of MS in recent years. Thus, we focused on the investigation of OSM as an immune-dependent risk factor in disease patho-physiology in Isfahan RRMS patients. To measure the association between OSM plasma level in MS patients and the healthy subjects. To evaluate the correlation between the demographic info such as age, sex, EDSS, drugs, and OSM concentration in cases. Blood sample from 60 RRMS patients and 30 healthy subjects between 15 and 40 years was gathered on EDTA and the evaluation on OSM in their plasma was achieved by EASTBIOPHARMA enzyme-linked immunosorbent assay (ELISA) kit. The statistical analysis was performed utilizing SPSS software. Data showed a significant higher OSM plasma level in the cases than the healthy donors (P value<0.05). As the first step of studying IL-6 cytokine family in autoimmune diseases in Iranian population, we accomplished to approve the OSM active role in RRMS patients of Isfahan province. Further future investigation of IL-6 family such as “studying IL-6 in central nervous system (CNS) plaques”, “genetic polymorphisms in association with MS”, and “revealing newer aspects of their implications in cellular pathways” might result in development of immunotherapy and diagnosis in chronic autoimmune diseases of the CNS.