P0121 Inhibitory effect of adlay hull and testa ethanolic extracts on uterine leiomyoma cell proliferation

Abstract

Background Uterine leiomyoma is a significant health problem. The associated symptoms can lead to reduced quality of life and women ultimately seek surgical intervention. Methods Adlay extract possesses anti-tumour activity and has shown to inhibit sarcoma cell proliferation and tumour progression. In the present study, viability of ELT3 cells was tested to screen for the different fractions of adlay extracts, as well as various phenolic compounds, flavonoids, phytosterols, and fatty acids. The active adlay fractions and compounds were then tested on primary human leiomyoma and myometrial smooth muscle cells. Cell cycle profiles of ELT3 cells were analysed to determine the possible cause of growth inhibition. Findings Results showed that AHE-EA, ATE-Hex, and ATE-EA were able to inhibit the proliferation of ELT3 and primary leiomyoma cells. Quercetin, nobiletin, eriodictyol, sitosterol, stigmasterol, and stigmastanol were among the tested compounds that significantly decreased the viability of ELT3 and primary leiomyoma cells, while linoleic acid only impeded primary leiomyoma cell growth. Eriodictyol and quercetin induced cell cycle arrest of ELT3 cells at sub-G1 and G2/M phases. Interpretation Adlay extract exhibits time-dependent and concentration-dependent growth-inhibitory effects on uterine leiomyoma cells. These effects could be caused by the combined actions of the different compounds, indicating the possibility of a matrix effect from the compounds in the adlay extract.