Abstract
The authors have found out the anti-P1+P+Pk antibodies in the serum of a patient who was given two times a small quantity of blood transfusion each. The antibodies consisted of complete and incomplete hemagglutinins and hemolysin. The blood groups and types of the patient were A, MNSs, CcDEe (Rh1 Rh2), kk, Fy (a+b-), Jk (a+b+), p (Tj [a-]), Le (a-). (Moreover the red blood cells of the patient had no Pk antigen.) The hemolysin was disappeared after 4 months odd since the first blood transfusion, but the agglutinins were not disappeared. (The incomplete agglutinin titre was on slight increase for a time after the transfusions.) The agglutininating antibodies not only was eluated in Ig G fraction, but also eluated in Ig A and Ig M fractions by DEAE-cellulose chromatography.The patient had a successful surgical operation without blood transfusion after recovery of anemia because there was no compatible blood donor in any patient kin and anyone of other volunteer in Hiroshima Prefecture. The agglutinating antibodies were reaffirmed by Dr. M. Yokoyama (Kuakini Research Institute, Honolulu, U. S. A.).
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