Abstract
Abstract Four different soluble components were identified by zonal centrifugation of adenovirus type 4 material. Listed in order of decreasing sedimentation rates they were: complete hemagglutinin (HA), group-specific complement-fixation (CF) antigen, rapidly and slowly sedimenting incomplete HA. The rapidly sedimenting incomplete HA was demonstrable in the presence of antisera against members of both Rosen's subgroup I (types 3 and 11) and subgroup III (types 2 and 5). In contrast the slowly sedimenting incomplete HA was detectable only in the presence of antibodies against members of Rosen's subgroup III. Incubation of complete HA at 56° led to a degradation into incomplete HA's of both kinds. Trypsin treatment did not affect complete HA and slowly sedimenting incomplete HA, but converted rapidly sedimenting incomplete HA into the slowly sedimenting form. The nature of the different components are discussed in the light of previously presented data on sedimentation characteristics of soluble components of adenovirus type 3 and (as a reference in the present study) adenovirus type 5. It is suggested that the complete HA might represent a symmetrical aggregate of 12 penton antigens, the group-specific CF component hexon antigen, the rapidly sedimenting incomplete HA-isolated penton antigen, and the slowly sedimenting incomplete HA fiber antigen.
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