P-466 Decellularization of whole organ human cervix: Physio and biochemical aspects of decellularized extracellular matrix

Abstract

Listen

Translate article

Abstract Study question Can the whole organ-cervix be decellularized while maintaining its fiber ultrastructure and extracellular matrix (ECM) functionality? Summary answer Cervix after decellularization could maintain the physical properties, ECM was found to be intact and functional. What is known already ECM-rich bioscaffolds are obtained from the process that utilizes the decellularization of tissues and organs. Cervical bioengineering is needed for clinical cervicovaginal reconstruction and tissue constructs can help women with cervical abnormalities. Study design, size, duration Human cervices were obtained from hysterectomized samples from 3 women undergoing surgery for complete hysterectomy during January 2021 to December 2021 Participants/materials, setting, methods Human cervices were obtained from hysterectomized samples from 3 women undergoing surgery and were subjected to whole organ decellularization. Various physical, chemical methods were employed to remove cellular remnants for a period of 10 days via perfusion of ionic and detergent solutions. Validation of complete decellularization was done via hematoxylin and eosin (H&E) staining, DNA quantification. ECM functionality was observed. Main results and the role of chance Well-organized ECM was obtained. Effective complete decellularization was dictated and verified by cervical tissue density, histopathological studies, DNA quantification and biochemical tests. H&E staining reflected the absence of any nuclear material in the endocervical and ectocervical tissues. Tissue microarchitecture and descending uterine artery were intact and could be helpful in the nutrient and gas exchange. DNA was found to be around 70 ng/mg tissue weight. Cell-free tissue-derived scaffolds were obtained along with a combination of growth factors and proteins. Limitations, reasons for caution Acellular cervical scaffold as a whole organ with vasculature intact for site-specific transplantation is a bigger question and still unaddressed. Wider implications of the findings Our study creates numerous prospects for in-vitro and in-vivo whole cervix tissue engineering. Site-specific tissue holds a promising avenue for the reconstruction of cervical abnormalities. Trial registration number Not applicable