P.1.g.077 The protective role of arylpiperazine in neuroinflammation

Abstract

Objetive: The purpose of the present work was to study the effects of standardized extract of Erythrina velutina (SEEV) in the determination of amino acids in mice brain undergoing global cerebral ischemia (ISQ). Methods: For this purpose, animals (swiss mice, males, 30−35 g) were subjected to transient cerebral ischemia by occlusion of both carotid arteries during 30 minutes and treated for 5 days with SEEV 200 or 400mg/kg and Memantine (MEM) 10mg/kg, 2 or 24 hours (2 or 24 h) after ischemia. The same procedure was done in false-operated group (FO) + dimethylsulfoxide (DMSO) with the exception of clamping the carotid arteries. On day 6 after induction of ischemia, the animals were subjected to sacrificed and dissected brains on ice the prefrontal cortex (PFC), determination of glutamate (GLU), and gammaamino-butirico acid (GABA). Statistical analysis were performed using two-way ANOVA considering significant when p< 0.05. Results: The results show there was an increase in the amount of glutamate in the PFC of ischemic animals treated with DMSO (ISQ + DMSO: 25.6±4.6) compared to the control group (FO + DMSO: 3.28±0.9). The amount GLU was reduced by both doses of SEEV (ISQ + SEEV 200mg 24 h: 21.9±6.1) and (ISQ + SEEV 400mg 24 h: 9.6±2.1) with only 24 hours. Regarding the inhibitory amino acid in the PFC groups (ISQ + SEEV 200mg 24 h: 355.7±71.0) and (ISQ + SEEV 400mg 2 h: 606.8±45.1) showed a significant increase when compared to ischemic control. Groups (ISQ + SEEV 200mg 2 h: 263.5±37.2), (ISQ + SEEV 400mg 2 h: 235.1±52.8), (ISQ + MEM 10mg 24 h: 300.4±49.7) showed an increase in GABA concentrations compared with the control group (ISQ + DMSO: 115.6±27.4). An increase in the concentrations of this amino acid in relation to time of administration of the drug can also be seen when comparing the group (ISQ + MEM 10mg 2 h: 138.0±21.8) and (ISQ + MEM 10mg 24 h: 300.4±49.7). Conclusion: In conclusion, these results corroborate the SEEV at both doses developed a neuroprotective action, possibly by reducing the levels of excitatory amino acids and increase inhibitory amino acids after on cerebral ischemia in mice.