Ozone oxidative intervention protects against hepatic ischemia reperfusion injury in rats

Abstract

Objective To explore the protective effect of ozone oxidative preconditioning on hepatic ischemia reperfusion injury in rats. Methods Eighteen 8-week-old SPF Sprague-Dawley male rats weighting 250~300 g were randomly divided into three groups (n=6 each): sham operation group (group S), ischemia/reperfusion group (group I/R) and ozone oxidative preconditioning group (group O3+ I/R). In Group O3+ I/R, rats received five-day preconditioning treatments by intraperitoneal injection of ozone and oxygen mixed gases (ozone concentration 50 mg/L, 1 mg·kg-1·d-1), and then experienced the procedure of hepatic ischemia/reperfusion injury. Model of hepatic ischemia/reperfusion injury was established by clamping the branches of hepatic artery and portal vein in the median and left lateral hepatic lobes for 45 min, followed by 3-h reperfusion. After reperfusion, blood samples were taken from the aorta abdominalis for detecting serum aminotransferases (ALT & AST). These rats were executed and the hepatic tissue samples were collec-ted for measuring hepatic malondialdehyde (MDA) and superoxide dismutase (SOD) level. Results Compared with group S, concentrations of serum ALT, AST and hepatic MDA were increased in group I/R and O3+ I/R; concentrations of hepatic SOD were decreased (P<0.05) in group I/R, but concentration of hepatic SOD was increased in group O3+ I/R. Compared with group IR, concentrations of serum ALT, AST and hepatic MDA were decreased, while concentration of hepatic SOD was increased in group O3+ I/R (P<0.05). Conclusion Ozone oxidative preconditioning could inhibit the lipid peroxidation to protect the rats against hepatic ischemia/reperfusion injury. Key words: Ozone oxidative preconditioning; Ischemia/reperfusion injury; Liver