Ozone Effects on Botrytis cinerea Conidia using a Bubble Column: Germination Inactivation and Membrane Phospholipids Oxidation

Abstract

Ozone treatments were applied on conidia aqueous suspensions in order to determine the minimal applied ozone dose to limit conidia germination and to observe the mechanisms involved in the spores inactivation. Conidia germination was significantly reduced, bubbling for at least 0.5 min as a gas with a minimal ozone concentration of 1 g.m−3. The applied ozone doses induce the membrane phospholipids oxidation, determined by the malondialdehyde quantification. Membrane phospholipids oxidation and inactivation rate are correlated. So, lipid peroxidation and consequently the alteration of the membrane integrity are involved in the antifungal action of ozone.