Effect of ozone stress on the intracellular metabolites from Cobetia marina

Junjie Li,Axel Rosenhahn,Oliver J Schmitz,Christoph Rumancev,Torsten C Schmidt,Holger V Lutze

doi:10.1007/s00216-020-02810-6

Junjie Li, Axel Rosenhahn + Show 4 more

Open Access

https://doi.org/10.1007/s00216-020-02810-6

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Abstract

A GCxGC-MS system was employed with a non-polar × mid-polar column set for the metabolic non-target analysis of Cobetia marina, the model bacteria for marine biofouling. C. marina was treated with ozone to investigate the intracellular metabolic state change under oxidative stress. A minimal inhibitory concentration test was involved to guarantee that the applied ozone dosages were not lethal for the cells. In this study, non-target analyses were performed to identify the metabolites according to the NIST database. As a result, over 170 signals were detected under normal living conditions including 35 potential metabolites. By the comparison of ozone-treated and non-treated samples, five compounds were selected to describe observed trends of signals in the contour plots. Oleic acid exhibited a slight growth by increasing ozone dosage. In contrast, other metabolites such as the amino acid l-proline showed less abundance after ozone treatment, which was more evident once ozone dosage was raised. Thus, this work could provide a hint for searching for up/downregulating factors in such environmental stress conditions for C. marina.Graphical abstract

Highlights

Microorganisms could optimally survive and reproduce due to the adaption to the normal environments [1]
Ozone-containing gas was produced onsite with an ozone generator (BMT 802 X, BMT Messtechnik, Berlin, Germany; feed gas: O2 6.0, Linde, Duesseldorf, Germany), which was bubbled into ice-cooled ultrapure water as shown in Fig. S1 in the Electronic Supplementary Material (ESM). 10 mM of indigotrisulfonate purchased from Sigma was dissolved in ultrapure water
The repeatability of the bacterial sample preparation method was investigated for different culture volume groups (5 mL, 10 mL, 30 mL) in triplicates with OD600 ≈ 1 in the stationary phase

Summary

Introduction

Microorganisms could optimally survive and reproduce due to the adaption to the normal environments [1]. Once passing through the membrane, it leads to DNA or intracellular protein damages, which impacts the reparation and transcription and might result in cell lysis or death [16, 17]

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