Abstract
Many research studies are directed toward developing safe and efficient collagen-based biomaterials as carriers for drug delivery systems. This article presents a comparative study of the properties of new collagen sponges prepared and characterized by different methods intended for biomedical applications. The structural integrity is one of the main properties for a biomaterial in order for it to be easily removed from the treated area. Thus, the effect of combining a natural polymer such as collagen with an antimicrobial drug such as oxytetracycline or doxycycline and glutaraldehyde as the chemical cross-linking agent influences the cross-linking degree of the material, which is in direct relation to its resistance to collagenase digestion, the drug kinetic release profile, and in vitro biocompatibility. The enzymatic degradation results identified oxytetracycline as the best inhibitor of collagenase when the collagen sponge was cross-linked with 0.5% glutaraldehyde. The drug release kinetics revealed an extended release of the antibiotic for oxytetracycline-loaded collagen sponges compared with doxycycline-loaded collagen sponges. Considering the behavior of differently prepared sponges, the collagen sponge with oxytetracycline and 0.5% glutaraldehyde could represent a viable polymeric support for the prevention/treatment of infections at the application site, favoring tissue regeneration.
Highlights
Antimicrobial resistance has become a major problem in recent years, and many studies in medicine are focused on this topic
More information [44,45] about the collagen sponges obtained can be extracted from Infrared spectroscopy (IR) spectra as follows: (a) hydrolysis degree calculated as the ratio of the band intensities AOH/AI (A~3300/A~1630 cm−1 ); (b) cross-linking degree obtained by the ratio of the band intensities AI/AOH (A~1630/A~3300 cm−1 ), high values of this ratio indicating a high cross-linking degree; (c) denaturation process evidenced by Equation (4), where νI and νII represents the wavenumbers of Amide I and Amide II, respectively; for ∆ν < 100, no distortion takes place
We presented a comparative study concerning the properties of new sponges prepared and characterized by numerous methods to provide their use as carrier supports in biomedical applications
Summary
Antimicrobial resistance has become a major problem in recent years, and many studies in medicine are focused on this topic Antibiotics are used both in human and veterinary therapies for infections reduction, and the role of resistance monitoring is very important [1,2]. The other tetracyclines were identified later, such as doxycycline hydrochloride (DXC) [4], which came into human pharmacological use in 1967. Due to their broad spectrum of action, OTC and DXC (Figure 1) are antimicrobial substances with bacteriostatic activity that are indicated for the treatment of some infections caused by Gram-negative and Gram-positive microorganisms [5]. Tetracyclines are effective against most spirochetes and anaerobic bacteria; this action is relevant in terms of periodontitis [6,7,8,9]
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