Abstract
Oxygen uptake and fructolysis of turkey spermatozoa in two artificial media have been studied. At optimum dilutions, spermatozoa respiring in phosphate buffer (pH 6.8) have a Z02 of 3, those in Earle's solution (pH 8.3) a Z02 of 7. Over a range from 0.3 to 3.5 billion cells per milliliter, respiration of spermatozoa in phosphate buffer is independent of dilution; oxygen uptake of sperm respiring in Earle's solution during 2 hours is quite sensitive to dilution. It is shown that decline of oxygen uptake of a sperm suspension with time proceeds independently of progressive death of the cells but is positively correlated with number of motile forms present. The fructolysis index is low (< 0.1) and fructolysis continues after cessation of motility. It is concluded that neither oxygen uptake nor fructolysis is likely to furnish a means for assessing fertilizing capacity of a semen sample.
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