Abstract
1. 1. The oxygen uptake of the L and R isolates of Trypanosoma lewisi originating in the United States were compared by conventional manometric technics. A standard number of cells (5 × 10 6) was employed in Warburg flasks (13–17 ml); a variable number of cells was also used as further check on observations. A comparison of the influence of crowding was also made of the L isolate. 2. 2. The L cell showed consistently greater oxygen uptake endogenously and with glucose and rat serum as substrates. Both isolates exhibited a progressively mounting pattern of oxygen consumption correlated with age of the infection but not related to the development of populations in the blood stream. 3. 3. The R form developed significantly greater populations in the blood stream of the rat. 4. 4. L cells at populations of 50 × 10 6 cells/flask showed 50 per cent inhibition of O 2 uptake with reference to the 5 × 10 6 cell level and 70 per cent inhibition with respect to the 500 × 10 6 population. Inhibition of respiratory activity progressively mounted with the age of the infection.
Published Version
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