Abstract
Surface plasmon resonance (SPR) is a label-free and real-time detection method that makes sensing with high sensitivity and without needing to label biomolecules. SPR biosensors are one of the standard analytical tools in the field of medicine and the pharmaceutic industry to study and analyze the interaction between target molecules and biological detection. A new dissolved oxygen sensor based on surface plasmon resonance (SPR) and Goos–Hanchen (GH) shift was proposed and demonstrated in immobilized hemoglobin on the gold layer that was coated on the SPR chip. Deoxyhemoglobin is converted to oxyhemoglobin when sodium bicarbonate is added to phosphate-buffered saline (PBS). The sensitivity of this sensor is related to the ratio of dissolved carbon dioxide to dissolved oxygen. The experimental results show that the difference in maximum GH shift between oxyhemoglobin and deoxyhemoglobin is about 30 μm with good repeatability. Therefore, these preliminary results showed the potential application of Goos-Hänchen enhanced plasmon resonance as a sensitive approach for recognizing oxygen-dissolved hemoglobin.
Published Version
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