Abstract
The toxicity of CuO nanoparticles (CuO NPs) to human hepatocarcinoma (HepG2) cells was investigated in this study. CuO NPs (1 - 40 mg/L) had significant toxicity to HepG2 cells. The antioxidant N-acetyl-L-cysteine (NAC) significantly reduces the cytotoxicity induced by the CuO NPs, supporting the hypothesis that oxidative stress contributes to the cytotoxicity of CuO NPs. To further explore the oxidative mechanisms of cytotoxicity, we examined CuO NPs-induced production of reactive oxygen species (ROS) in HepG2 cells. CuO NPs generated intracellular ROS in HepG2 cells in a concentration-dependent manner.
Highlights
Metal and metal oxide nanoparticles (NPs) are widely applied in personal care products [1], catalysis [2], optical and recording devices [3], and water purification [4]
How to cite this paper: Fu, X. (2015) Oxidative Stress Induced by CuO Nanoparticles (CuO NPs) to Human Hepatocarcinoma (HepG2) Cells
Karlsson et al reported that CuO NPs were significantly more toxic than ZnO, Fe2O3, TiO2 NPs and CuO NPs resulted in oxidative stress for human cells [23]
Summary
Metal and metal oxide nanoparticles (NPs) are widely applied in personal care products [1], catalysis [2], optical and recording devices [3], and water purification [4]. The toxicity of metal and metal oxide NPs on mammalian cells has been studied, and a number of researchers have found that metal and metal oxide NPs can generate oxidative stress and promote cytotoxicity [7] [8]. Recent studies have shown the oxidative stress mediated toxicity of CuO NPs in different human cell lines, such as human lung epithelial (A549), human cardiac microvascular endothelial, kidney and neuronal cells. (2015) Oxidative Stress Induced by CuO Nanoparticles (CuO NPs) to Human Hepatocarcinoma (HepG2) Cells. Wang et al [17] observer that cytoxicity of CuO NPs in HepG2 cells was found the relationship between reactive oxygen species (ROS) generation and gene expression during CuO NPs exposure. Karlsson et al reported that CuO NPs were significantly more toxic than ZnO, Fe2O3, TiO2 NPs and CuO NPs resulted in oxidative stress for human cells [23]
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