Oxidative DNA damage in female patients with diabetes mellitus type 2.

Abstract

Event Abstract Back to Event Oxidative DNA damage in female patients with diabetes mellitus type 2. Bianca Guggenberger1, Annemarie Grindel1*, Carina Kern1, Helmut Brath2 and Karl-Heinz Wagner1 1 University of Vienna, Department of Nutritional Sciences, Austria 2 Diabetes Outpatient Clinic, Health Centre South, Austria Diabetes mellitus type 2 is very often associated with increased levels of reactive oxygen species. Previous studies also showed higher DNA damage in patients with diabetes mellitus type 2 compared to healthy controls (Tatsch et al., 2012; Blasiak et al., 2004). However, blood glucose concentration and level of glycated hemoglobin (HbA1c) can vary widely between diabetic patients and depends on country specific threshold levels. Therefore our aim was to analyze DNA damage in patients with lower glycated hemoglobin (HbA1c<7.5%; n=74) vs. patients with higher glycated hemoglobin (HbA1c>7.5%; n=72). In total 146 patients of the local diabetes outpatient clinic in Vienna were enrolled (age: 67.5±9,9years; BMI: 35.0±7.6kg/m²; diabetes duration: 14.4±8.0years).The Comet assay was performed in PBMCs (strand breaks, resistance to H2O2, FPG-sensitive sites) and whole blood (strand breaks, FPG-sensitive sites). No differences in DNA damage were found between the two groups of low vs. high HbA1c. In addition, neither diabetes duration nor medication (Insulin vs. oral antidiabetics) had an influence on DNA damage. Therefore we can conclude that female patients with diabetes mellitus type 2 in Austria are under optimal treatment to control blood sugar and other metabolic parameter, that no differences in DNA damage could be observed. References Tatsch, E., G. V. Bochi, S. J. Piva, J. A. M. De Carvalho, H. Kober, V. D. Torbitz, T. Duarte, C. Signor, A. C. Coelho, M. Duarte, G. Montagner, I. B. M. Da Cruz and R. N. Moresco (2012). "Association between DNA strand breakage and oxidative, inflammatory and endothelial biomarkers in type 2 diabetes." Mutation Research-Fundamental and Molecular Mechanisms of Mutagenesis 732(1-2): 16-20. Blasiak, J., M. Arabski, R. Krupa, K. Wozniak, M. Zadrozny, J. Kasznicki, M. Zurawska and J. Drzewoski (2004). "DNA damage and repair in type 2 diabetes mellitus." Mutation Research-Fundamental and Molecular Mechanisms of Mutagenesis 554(1-2): 297-304. Keywords: Diabetes Mellitus, Type 2, Comet Assay, Glycated hemoglobin, PBMC, whole blood Conference: ICAW 2015 - 11th International Comet Assay Workshop, Antwerpen, Belgium, 1 Sep - 4 Sep, 2015. Presentation Type: Poster Presentation Topic: Clinical applications of the comet assay Citation: Guggenberger B, Grindel A, Kern C, Brath H and Wagner K (2015). Oxidative DNA damage in female patients with diabetes mellitus type 2.. Front. Genet. Conference Abstract: ICAW 2015 - 11th International Comet Assay Workshop. doi: 10.3389/conf.fgene.2015.01.00030 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 02 May 2015; Published Online: 23 Jun 2015. * Correspondence: Ms. Annemarie Grindel, University of Vienna, Department of Nutritional Sciences, Vienna, Austria, annemarie.grindel@univie.ac.at Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Bianca Guggenberger Annemarie Grindel Carina Kern Helmut Brath Karl-Heinz Wagner Google Bianca Guggenberger Annemarie Grindel Carina Kern Helmut Brath Karl-Heinz Wagner Google Scholar Bianca Guggenberger Annemarie Grindel Carina Kern Helmut Brath Karl-Heinz Wagner PubMed Bianca Guggenberger Annemarie Grindel Carina Kern Helmut Brath Karl-Heinz Wagner Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.